描述

The PureLink® RNA Mini Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol. Obtain high-yield, high-integrity RNA from animal and plant cells and tissues as well as blood, bacteria, yeast, and liquid samples. The PureLink® RNA Mini Kit provides:

• Rapid, non-hazardous RNA purification capabilities
• Ready-to-use RNA for many downstream applications

Rapid and convenient column purification 
The PureLink® RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and tissue types to yield up to 1,000 µg of purified RNA from a single extraction (see table). High-quality total RNA can be obtained from mini- to midi-prep amounts of starting material with no genomic DNA contamination.

Simplified, nontoxic RNA purification
The PureLink® RNA Mini Kit combines nontoxic guanidine-isothiocyanate lysis with the speed, purity, and ease-of-use of silica-membrane purification. The safe and easy procedure can typically be completed in less than 20 minutes without the need for hazardous phenol/chloroform extraction, CsCl centrifugation, LiCl, or alcohol precipitation.

規格

Sample Type (General):Bacteria, Blood, Tissue, Plant Samples, Cells, Yeast
High Throughput Compatibility:Not High Throughput-Compatible (Manual)
Downstream Application:Reverse Transcriptase PCR (RT-PCR), Real-Time Quantitative PCR (qPCR), Microarray Analysis, Next-Generation Sequencing, Nuclease Protection Assays, cDNA Library Construction, Northern Blotting
Scale:Mini
System:PureLink™
Quantity:10 columns
Product Size:10 preps
Final Product:Total RNA
Yield (up to):1000 µg
Number of Reactions:10 Preps
Elution Volume - max:300 µl
Elution Volume - min:30 µl
Isolation Technology:Silica Spin Column
Starting Material (Amount):Up to 200 mg tissue, 5 x 10^6 to 1 x 10^8 cells
Shipping Condition:Room Temperature

内容及儲存

The PureLink® RNA Mini Kit comes with enough RNA lysis solution, spin cartridges, wash tubes, recovery tubes, wash buffers, and RNase-free water to perform 10 RNA purifications. All components should be stored at room temperature. Guaranteed stable for 1 year when properly stored.